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Chemical analysis of in vivo–irradiated dentine of head and neck cancer patients by ATR-FTIR and Raman spectroscopy

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Abstract

Objectives

To evaluate the effect of in vivo radiotherapy on the chemical properties of human dentine by Fourier-transform infrared spectroscopy (FTIR) and Raman analysis.

Materials and methods

Chemical composition was evaluated comparing control and irradiated group (n = 8). Irradiated teeth were obtained from radiotherapy patients subjected to fractionated X-ray radiation of 1.8 Gy daily totaling 72 Gy. The teeth were sectioned according to the type of dentine (crown or root dentine), obtaining 3-mm dentine cervical slices. The analyzed parameters by FTIR and Raman spectroscopies were mineral/matrix ratio (M:M), carbonate/mineral ratio (C:M), amide I/amide III ratio, and amide I/CH2 ratio. Raman also calculated the phosphate and carbonate crystallinity.

Results

FTIR revealed that M:M had a decrease in both factors (p = 0.008; p = 0.043, respectively) and root dentine showed a lower C:M in the irradiated group (p = 0.003). Raman revealed a higher phosphate crystallinity and a lower carbonate crystallinity in crown dentine of irradiated group (p = 0.021; p = 0.039). For amide I/amide III, the irradiated showed a lower ratio when compared to the control group (FTIR p = 0.002; Raman p = 0.017). For amide I/CH2, the root dentine showed a higher ratio than the crown dentine in both methods (p < 0.001).

Conclusions

Radiotherapy altered the chemical composition of human dentine. The exchange of phosphate-carbonate ions in the hydroxyapatite and higher concentration of organic components was found after radiotherapy.

Clinical relevance

The increased risk of radiation-related caries in patients undergoing head and neck radiotherapy is due not only to salivary, dietary, and microbiological changes but also to changes in tooth chemical composition.

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Acknowledgements

The authors thank FAPEMIG, CNPq and CAPES (Brazil) for the financial support. The authors are also grateful to the Laboratory of New Insulating Materials and Semiconductors (LNMIS) at Institute of Physics, Federal University of Uberlândia, Minas Gerais, Brazil.

Funding

The study was supported by FAPEMIG, CNPq and CAPES for the granting of scholarship and resource for the purchase of consumer material.

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Authors and Affiliations

  1. Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Av Pará 1720, Block 4L, room A32, Campus Umuarama, Uberlândia, Minas Gerais, 38405-320, Brazil

    Rafael Resende de Miranda, Carlos José Soares & Veridiana Resende Novais

  2. Laboratory of New Nanostructured and Functional Materials, Institute of Physics, Federal University of Alagoas, Av Lourival Melo Mota S/N, Campus A. C. Simões, Maceió, Alagoas, 57072-970, Brazil

    Anielle Christine Almeida Silva & Noelio Oliveira Dantas

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  1. Rafael Resende de Miranda
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  2. Anielle Christine Almeida Silva
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Correspondence to Veridiana Resende Novais.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study. Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.

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de Miranda, R.R., Silva, A.C.A., Dantas, N.O. et al. Chemical analysis of in vivo–irradiated dentine of head and neck cancer patients by ATR-FTIR and Raman spectroscopy. Clin Oral Invest 23, 3351–3358 (2019). https://doi.org/10.1007/s00784-018-2758-6

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  • DOI: https://doi.org/10.1007/s00784-018-2758-6

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